By Chirag Mandavia
In order to review AT2-mediated sign transduction mechanisms, major methods have been used: (1) the ligand-binding homes of mutant AT2 receptors have been characterised compared to wild-type after iteration of some extent mutation utilizing site-directed mutagenesis, and (2) interacting protein companions have been analyzed utilizing yeast library screening, co-immunoprecipitation and Western Blot reviews. We stumbled on that the power of Angiotensin II (Ang II) receptor sort AT2 Phe308Ala residue mutant within the seventh TMD to bind peptidic ligands used to be tremendously lowered, utilizing Xenopus oocytes expression approach. this sediment occupies a place within the AT2 analogous to the Tyr292 within the AT1 crucial for agonistic activation of that receptor. Screening of a 17-day mouse embryo cDNA library with the Angiotensin II receptor AT2 because the bait in yeast two-hybrid assay pointed out sodium-hydrogen exchanger NHE6 because the binding associate with ninety eight% series homology. additional experiences printed that once human cell-line MCF-7 used to be transfected with the AT2, the AT2 co-immunoprecipitated with the NHE6 within the presence of Ang II, implying that Ang II-mediated activation is required for the interplay among AT2 and NHE6.
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Characterization of the Angiotensin receptor AT2 by various studies by Chirag Mandavia